Détail du document
Identifiant
oai:pubmedcentral.nih.gov:8677...
Sujet
ArticleAuteur
Chivukula, Sudha Plitnik, Timothy Tibbitts, Timothy Karve, Shrirang Dias, Anusha Zhang, Donghui Goldman, Rebecca Gopani, Hardip Khanmohammed, Asad Sarode, Ashish Cooper, Dustin Yoon, Heesik Kim, Younghoon Yan, Yanhua Mundle, Sophia T. Groppo, Rachel Beauvais, Adrien Zhang, Jinrong Anosova, Natalie G. Lai, Charles Li, Lu Ulinski, Gregory Piepenhagen, Peter DiNapoli, Joshua Kalnin, Kirill V. Landolfi, Victoria Swearingen, Ron Fu, Tong-Ming DeRosa, Frank Casimiro, DaniloLangue
enEditeur
Nature Publishing Group UK
Catégorie
PMC full-text journals
Année
2021
Date de référencement
13/12/2022
Mots clés
ha na viral development vaccine influenzaRésumé
Recent approval of mRNA vaccines for emergency use against COVID-19 is likely to promote rapid development of mRNA-based vaccines targeting a wide range of infectious diseases.
Compared to conventional approaches, this vaccine modality promises comparable potency while substantially accelerating the pace of development and deployment of vaccine doses.
Already demonstrated successfully for single antigen vaccines such as for COVID-19, this technology could be optimized for complex multi-antigen vaccines.
Herein, utilizing multiple influenza antigens, we demonstrated the suitability of the mRNA therapeutic (MRT) platform for such applications.
Seasonal influenza vaccines have three or four hemagglutinin (HA) antigens of different viral subtypes.
In addition, influenza neuraminidase (NA), a tetrameric membrane protein, is identified as an antigen that has been linked to protective immunity against severe viral disease.
We detail the efforts in optimizing formulations of influenza candidates that use unmodified mRNA encoding full-length HA or full-length NA encapsulated in lipid nanoparticles (LNPs).
HA and NA mRNA-LNP formulations, either as monovalent or as multivalent vaccines, induced strong functional antibody and cellular responses in non-human primates and such antigen-specific antibody responses were associated with protective efficacy against viral challenge in mice.
Chivukula, Sudha,Plitnik, Timothy,Tibbitts, Timothy,Karve, Shrirang,Dias, Anusha,Zhang, Donghui,Goldman, Rebecca,Gopani, Hardip,Khanmohammed, Asad,Sarode, Ashish,Cooper, Dustin,Yoon, Heesik,Kim, Younghoon,Yan, Yanhua,Mundle, Sophia T.,Groppo, Rachel,Beauvais, Adrien,Zhang, Jinrong,Anosova, Natalie G.,Lai, Charles,Li, Lu,Ulinski, Gregory,Piepenhagen, Peter,DiNapoli, Joshua,Kalnin, Kirill V.,Landolfi, Victoria,Swearingen, Ron,Fu, Tong-Ming,DeRosa, Frank,Casimiro, Danilo, 2021, Development of multivalent mRNA vaccine candidates for seasonal or pandemic influenza, Nature Publishing Group UK
