Détail du document
Identifiant
oai:HAL:hal-04272832v1
Sujet
RT-LAMP highly pathogenic avian influenza ... clade 2.3.4.4b molecular detection... clinical diagnostic field detection [SHS.STAT]Humanities and Social Sc... [SDV.BA.MVSA]Life Sciences [q-bio]... [SDV.MP]Life Sciences [q-bio]/Micr... [SDV.MP.VIR]Life Sciences [q-bio]/...Auteur
Filaire, Fabien Sécula, Aurélie Lebre, Laetitia Croville, Guillaume Guerin, Jean-LucLangue
enEditeur
HAL CCSD;Taylor & Francis
Catégorie
sciences : sciences du vivant
Année
2023
Date de référencement
15/12/2023
Mots clés
highly avian influenza pathogenic [sdv sciences clinical 4b clade hpaiv h5 assay rt-lampRésumé
International audience; Highly pathogenic avian influenza viruses (HPAIV) are a major threat to the global poultry industry and public health due to their zoonotic potential.
Since 2016, Europe and France have faced major epizootics caused by clade 2.3.4.4b H5 HPAIV.
To reduce sample-to-result times, point-of-care testing is urgently needed to help prevent further outbreaks and the propagation of the virus.
This study presents the design of a novel real-time colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of clade 2.3.4.4b H5 HPAIV.
A clinical validation of this RT-LAMP assay was performed on 198 pools of clinical swabs sampled in 52 pvirusoultry flocks during the H5 HPAI 2020-2022 epizootics in France.
This RT-LAMP assay allowed the specific detection of HPAIV H5Nx clade 2.3.4.4b within 30 minutes with a sensitivity of 86.11%.
This rapid, easy-to-perform, inexpensive, molecular detection assay could be included in the HPAIV surveillance toolbox.
Filaire, Fabien,Sécula, Aurélie,Lebre, Laetitia,Croville, Guillaume,Guerin, Jean-Luc, 2023, A real-time colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of highly pathogenic H5 clade 2.3.4.4b avian influenza viruses, HAL CCSD;Taylor & Francis
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