Document detail
ID
doi:10.1186/s13059-024-03262-2...
Author
Cui, Xiao-Long Nie, Ji Zhu, Houxiang Kowitwanich, Krissana Beadell, Alana V. West-Szymanski, Diana C. Zhang, Zhou Dougherty, Urszula Kwesi, Akushika Deng, Zifeng Li, Yan Meng, Danqing Roggin, Kevin Barry, Teresa Owyang, Ryan Fefferman, Ben Zeng, Chang Gao, Lu Zhao, Carolyn W. T. Malina, Yuri Wei, Jiangbo Weigert, Melanie Kang, Wenjun Goel, Ajay Chiu, Brian C.-H. Bissonnette, Marc Zhang, Wei Chen, Mengjie He, ChuanLangue
enEditor
BioMed Central
Category
Life Sciences
Year
2024
listing date
6/19/2024
Keywords
linear amplification bisulfite sequencing low-input dna cell-free dna cancer detection labs cancer dnaAbstract
Methylation-based liquid biopsies show promises in detecting cancer using circulating cell-free DNA; however, current limitations impede clinical application.
Most assays necessitate substantial DNA inputs, posing challenges.
Additionally, underrepresented tumor DNA fragments may go undetected during exponential amplification steps of traditional sequencing methods.
Here, we report linear amplification-based bisulfite sequencing (LABS), enabling linear amplification of bisulfite-treated DNA fragments in a genome-wide, unbiased fashion, detecting cancer abnormalities with sub-nanogram inputs.
Applying LABS to 100 patient samples revealed cancer-specific patterns, copy number alterations, and enhanced cancer detection accuracy by identifying tissue-of-origin and immune cell composition.
Cui, Xiao-Long,Nie, Ji,Zhu, Houxiang,Kowitwanich, Krissana,Beadell, Alana V.,West-Szymanski, Diana C.,Zhang, Zhou,Dougherty, Urszula,Kwesi, Akushika,Deng, Zifeng,Li, Yan,Meng, Danqing,Roggin, Kevin,Barry, Teresa,Owyang, Ryan,Fefferman, Ben,Zeng, Chang,Gao, Lu,Zhao, Carolyn W. T.,Malina, Yuri,Wei, Jiangbo,Weigert, Melanie,Kang, Wenjun,Goel, Ajay,Chiu, Brian C.-H.,Bissonnette, Marc,Zhang, Wei,Chen, Mengjie,He, Chuan, 2024, LABS: linear amplification-based bisulfite sequencing for ultrasensitive cancer detection from cell-free DNA, BioMed Central
Critical Prognostic Factors in Cerebral Venous Sinus Thrombosis: An Observational Study
thrombosis 001 p<0 involvement sinus prognostic study factors outcome poor associated
