doi:10.1007/s12022-023-09781-1...
Springer
Medicine & Public Health
2023
9/6/2023
Non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) is a low-risk thyroid tumor with a favorable prognosis.
Nonetheless, differentiating NIFTP from other thyroid tumors remains challenging, necessitating reliable diagnostic markers.
This study is aimed at discovering NIFTP-specific mRNA markers through RNA sequencing analysis of thyroid tumor tissues.
We performed mRNA expression profiling for 74 fresh frozen thyroid tissue samples, including NIFTP and benign and malignant follicular-cell-derived tumors.
NIFTP/malignant tumors showed 255 downregulated genes and 737 upregulated genes compared to benign tumors.
Venn diagram analysis revealed 19 significantly upregulated and 7 downregulated mRNAs in NIFTP.
Akaike information criterion analysis allowed us to select OCLN , ZNF423 , LYG1 , and AQP5 mRNA markers.
We subsequently developed a predictive model based on logistic regression analysis using these four mRNAs, which we validated in independent samples ( n = 90) using a qRT-PCR assay.
This model demonstrated high accuracy in predicting NIFTP in discovery dataset (AUC (area under the receiver operating characteristic) = 0.960) and the validation dataset (AUC = 0.757).
Our results suggest that OCLN , ZNF423 , LYG1 , and AQP5 mRNA markers might serve as reliable molecular markers for identifying NIFTP among other thyroid tumors, ultimately aiding in accurate diagnosis and management of NIFTP patients.
Lee, So-Yeon,Park, Jong-Lyul,Kim, Kwangsoon,Bae, Ja Seong,Kim, Jae-Yoon,Kim, Seon-Young,Jung, Chan Kwon, 2023, Identification of NIFTP-Specific mRNA Markers for Reliable Molecular Diagnosis of Thyroid Tumors, Springer