Document detail
ID
doi:10.1186/s12866-024-03258-9...
Author
Hu, Zhe Weng, Qiangang Cai, Zhehui Zhang, HuaweiLangue
enEditor
BioMed Central
Category
Mycology
Year
2024
listing date
4/10/2024
Keywords
chrysomycin marine fermentation optimization single-factor experiment response surface methodology yield strain 891-b6 conditions ca fermentationAbstract
Background Chrysomycin A (CA) is a promising antibiotic for treatment of Gram-positive bacterial infections and cancers.
In order to enhance CA yield, optimization of fermentation conditions and medium components was carried out on strain Streptomyces sp. 891-B6, an UV-induced mutant with improved CA titer compared with its wide-type marine strain 891.
Results Using one-way experiment, the optimal fermentation conditions for CA production in 1-L shake flask were obtained as follows: 12 days of fermentation time, 5 days of seed age, 5% of inoculum volume ratio, 200 mL of loading volume and 6.5 of initial pH.
By response surface methodology, the optimal medium components determined as glucose (39.283 g/L), corn starch (20.662 g/L), soybean meal (15.480 g/L) and CaCO_3 (2.000 g/L).
Conclusion Validation tests showed that the maximum yield of CA reached 1601.9 ± 56.7 mg/L, which was a 60% increase compared to the initial yield (952.3 ± 53.2 mg/L).
These results provided an important basis for scale-up production of CA by strain 891-B6.
Hu, Zhe,Weng, Qiangang,Cai, Zhehui,Zhang, Huawei, 2024, Optimization of fermentation conditions and medium components for chrysomycin a production by Streptomyces sp. 891-B6, BioMed Central
Evaluating Image Hallucination in Text-to-Image Generation with Question-Answering
evaluation computer dataset images tti generation i-halla
Role of Repetitive Transcranial Magnetic Stimulation in Treatment of Fibromyalgia: A Randomized Controlled Trial
significant following stimulation fms treatment
