Document detail
ID
oai:pubmedcentral.nih.gov:1079...
Topic
ArticleAuthor
Wang, James Seo, Jai Woong Kare, Aris J. Schneider, Martin Tumbale, Spencer K. Wu, Bo Raie, Marina N. Pandrala, Mallesh Iagaru, Andrei Brunsing, Ryan L. Charville, Gregory W. Park, Walter G. Ferrara, Katherine W.Langue
enEditor
Cold Spring Harbor Laboratory
Category
biorxiv
Year
2024
listing date
1/24/2024
Keywords
cancer claudin-4 markers spatialAbstract
We apply spatial transcriptomics and proteomics to select pancreatic cancer surface receptor targets for molecular imaging and theranostics using an approach that can be applied to many cancers.
Selected cancer surfaceome epithelial markers were spatially correlated and provided specific cancer localization, whereas the spatial correlation between cancer markers and immune- cell or fibroblast markers was low.
While molecular imaging of cancer-associated fibroblasts and integrins has been proposed for pancreatic cancer, our data point to the tight junction protein claudin-4 as a theranostic target.
Claudin-4 expression increased ∼16 fold in cancer as compared with normal pancreas, and the tight junction localization conferred low background for imaging in normal tissue.
We developed a peptide-based molecular imaging agent targeted to claudin-4 with accumulation to ∼25% injected activity per cc (IA/cc) in metastases and ∼18% IA/cc in tumors.
Our work motivates a new approach for data-driven selection of molecular targets.
Wang, James,Seo, Jai Woong,Kare, Aris J.,Schneider, Martin,Tumbale, Spencer K.,Wu, Bo,Raie, Marina N.,Pandrala, Mallesh,Iagaru, Andrei,Brunsing, Ryan L.,Charville, Gregory W.,Park, Walter G.,Ferrara, Katherine W., 2024, Spatial transcriptomic analysis drives PET imaging of tight junction protein expression in pancreatic cancer theranostics , Cold Spring Harbor Laboratory
