Influenza Virus RNA-Dependent RNA Polymerase and the Host Transcriptional Apparatus

Krischuns, Tim; Lukarska, Maria; Naffakh, Nadia; Cusack, Stephen

Influenza Virus RNA-Dependent RNA Polymerase and the Host Transcriptional Apparatus

Document detail

ID

oai:HAL:pasteur-03408583v1

Topic

RNAP II cap-snatching influenza polymerase nuclear cap-binding complex promoter-proximal pausing transcription [SDV.BBM.BM]Life Sciences [q-bio]/... [SDV.MP.VIR]Life Sciences [q-bio]/...

Author

Krischuns, Tim Lukarska, Maria Naffakh, Nadia Cusack, Stephen

Langue

Editor

HAL CCSD;Annual Reviews

Year

2021

listing date

9/29/2023

Keywords

cap-snatching influenza ii flupol rna polymerase rnap transcription

Metrics

Abstract

International audience; Influenza virus RNA-dependent RNA polymerase (FluPol) transcribes the viral RNA genome in the infected cell nucleus.

In the 1970s, researchers showed that viral transcription depends on host RNA polymerase II (RNAP II) activity and subsequently that FluPol snatches capped oligomers from nascent RNAP II transcripts to prime its own transcription.

Exactly how this occurs remains elusive.

Here, we review recent advances in the mechanistic understanding of FluPol transcription and early events in RNAP II transcription that are relevant to cap-snatching.

We describe the known direct interactions between FluPol and the RNAP II C-terminal domain and summarize the transcription-related host factors that have been found to interact with FluPol.

We also discuss open questions regarding how FluPol may be targeted to actively transcribing RNAP II and the exact context and timing of cap-snatching, which is presumed to occur after cap completion but before the cap is sequestered by the nuclear cap-binding complex.

Krischuns, Tim,Lukarska, Maria,Naffakh, Nadia,Cusack, Stephen, 2021, Influenza Virus RNA-Dependent RNA Polymerase and the Host Transcriptional Apparatus, HAL CCSD;Annual Reviews