oai:pubmedcentral.nih.gov:9530...
Frontiers Media S.A.
PMC full-text journals
2022
10/11/2022
BACKGROUND: Thyroid hormones (THs) inhibit hepatocellular carcinoma (HCC) through different mechanisms.
However, whether microRNAs play a role in the antitumorigenic effect of THs remains unknown.
METHODS: By next generation sequencing (NGS) we performed a comprehensive comparative miRNomic and transcriptomic analysis of rat hepatic preneoplastic lesions exposed or not to a short-term treatment with triiodothyronine (T3).
The expression of the most deregulated miRs was also investigated in rat HCCs, and in human hepatoma cell lines, treated or not with T3.
RESULTS: Among miRs down-regulated in preneoplastic nodules following T3, co-expression networks revealed those targeting thyroid hormone receptor-β (Thrβ) and deiodinase1, and Oxidative Phosphorylation.
On the other hand, miRs targeting members of the Nrf2 Oxidative Pathway, Glycolysis, Pentose Phosphate Pathway and Proline biosynthesis – all involved in the metabolic reprogramming displayed by preneoplastic lesions– were up-regulated.
Notably, while the expression of most miRs deregulated in preneoplastic lesions was not altered in HCC or in hepatoma cells, miR-182, a miR known to target Dio1 and mitochondrial complexes, was down-deregulated by T3 treatment at all stages of hepatocarcinogenesis and in hepatocarcinoma cell lines.
In support to the possible critical role of miR-182 in hepatocarcinogenesis, exogenous expression of this miR significantly impaired the inhibitory effect of T3 on the clonogenic growth capacity of human HCC cells.
CONCLUSIONS: This work identified several miRNAs, so far never associated to T3.
In addition, the precise definition of the miRNA-mRNA networks elicited by T3 treatment gained in this study may provide a better understanding of the key regulatory events underlying the inhibitory effect of T3 on HCC development.
In this context, T3-induced down-regulation of miR-182 appears as a promising tool.
Serra, Marina,Pal, Rajesh,Puliga, Elisabetta,Sulas, Pia,Cabras, Lavinia,Cusano, Roberto,Giordano, Silvia,Perra, Andrea,Columbano, Amedeo,Kowalik, Marta Anna, 2022, mRNA-miRNA networks identify metabolic pathways associated to the anti-tumorigenic effect of thyroid hormone on preneoplastic nodules and hepatocellular carcinoma, Frontiers Media S.A.