Genomic and phenotypic analysis of a novel clinical isolate of Corynebacterium pyruviciproducens

Background Corynebacterium pyruviciproducens is a recently described species of Corynebacterium .

There are few reports on the microbiological characteristics of the new species, and there is a lack of reports on the genomic analysis of the species.

Results This study involved a clinical isolate from the pus of a hospital patient with sebaceous gland abscesses.

The clinically isolated strain was identified as C. pyruviciproducens strain WYJY-01.

In this study, referring to Koch’s postulates, we observed the pathological changes of animal models infected by intraperitoneal injection and subcutaneous injection of pure culture of the strain WYJY-01.

Furthermore, the strain WYJY-01 was isolated and cultured again from animal models' subcutaneous abscess drainage fluid.

Subsequently, the genomics of the strain WYJY-01 was analyzed.

By comparing various gene databases, this study predicted the core secondary metabolite gene cluster of the strain WYJY-01, virulence factor genes carried by prophage, pathogenicity islands, and resistance islands.

In addition, the genomes of C. pyruviciproducens strain WYJY-01, ATCC BAA-1742^ T, and UMB0763 were analyzed by comparative genomics, and the differential genes of strain WYJY-01 were compared, and their functions were analyzed.

Conclusion The findings showed that the strain WYJY-01 had pathogenicity, supplementing the phenotype characteristics of C. pyruviciproducens .

Meanwhile, this research revealed the possible molecular mechanism of the pathogenicity of the strain WYJY-01 at the gene level through whole genome sequence analysis, providing a molecular basis for further research.