Document detail
ID
oai:pubmedcentral.nih.gov:8208...
Topic
Research ArticlesAuthor
Barroso, Shana Priscila Coutinho Vicente dos Santos, Ana Clara Souza dos Santos, Patrícia dos Santos Silva Couceiro, José Nelson Fernandes Ferreira, Davis Nico, Dirlei Morrot, Alexandre Lima Silva, Jerson Cheble de Oliveira, AndreaLangue
enEditor
Microbiology Society
Category
Access Microbiology
Year
2021
listing date
11/8/2022
Keywords
virus hydrostatic inactivation influenza pressureAbstract
Vaccines are a recommended strategy for controlling influenza A infections in humans and animals.
Here, we describe the effects of hydrostatic pressure on the structure, morphology and functional characteristics of avian influenza A H3N8 virus.
The effect of hydrostatic pressure for 3 h on H3N8 virus revealed that the particles were resistant to this condition, and the virus displayed only a discrete conformational change.
We found that pressure of 3 kbar applied for 6 h was able to inhibit haemagglutination and infectivity while virus replication was no longer observed, suggesting that full virus inactivation occurred at this point.
However, the neuraminidase activity was not affected at this approach suggesting the maintenance of neutralizing antibody epitopes in this key antigen.
Our data bring important information for the area of structural virology of enveloped particles and support the idea of applying pressure-induced inactivation as a tool for vaccine production.
Barroso, Shana Priscila Coutinho,Vicente dos Santos, Ana Clara,Souza dos Santos, Patrícia,dos Santos Silva Couceiro, José Nelson,Fernandes Ferreira, Davis,Nico, Dirlei,Morrot, Alexandre,Lima Silva, Jerson,Cheble de Oliveira, Andrea, 2021, Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach, Microbiology Society
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