Document detail
ID

oai:pubmedcentral.nih.gov:8485...

Topic
Research Article
Author
Mehrbod, Parvaneh Safari, Hanieh Mollai, Zeinab Fotouhi, Fatemeh Mirfakhraei, Yasaman Entezari, Hanieh Goodarzi, Saied Tofighi, Zahra
Langue
en
Editor

BioMed Central

Category

BMC Complementary Medicine and Therapies

Year

2021

listing date

12/4/2023

Keywords
plants influenza methanol leaves virus extracts fractions
Metrics

Abstract

BACKGROUND: Influenza A virus (IAV) infection is a continual threat to the health of animals and humans globally.

Consumption of the conventional drugs has shown several side effects and drug resistance.

This study was aimed to screen some Iranian medicinal plants extracts and their fractions against influenza A virus.

METHODS: Glycyrrhiza glabra (rhizome), Myrtus  commonis (leaves), Melissa officinalis (leaves), Hypericum perforatum (aerial parts), Tilia platyphyllos (flower), Salix alba (bark), and Camellia sinensis (green and fermented leaves) were extracted with 80% methanol and fractionated with chloroform and methanol, respectively.

The cytotoxicity of the compounds were determined by MTT colorimetric assay on MDCK cells.

The effective concentrations (EC(50)) of the compounds were calculated from the MTT results compared to the negative control with no significant effects on cell viability.

The effects of EC(50) of the compounds on viral surface glycoproteins and viral titer were tested by HI and HA virological assays, respectively and compared with oseltamivir and amantadine.

Preliminary phytochemical analysis were done for promising anti-IAV extracts and fractions.

RESULTS: The most effective samples against IAV titer (P ≤ 0.05) were crude extracts of G. glabra, M. officinalis and S. alba; methanol fractions of M. communis and M. officinalis; and chloroform fractions of M. communis and C. sinensis (fermented) mostly in co- and pre-penetration combined treatments.

The potential extracts and fractions were rich in flavonoids, tannins, steroids and triterpenoids.

CONCLUSION: The outcomes confirmed a scientific basis for anti-influenza A virus capacity of the extracts and fractions from the selected plants for the first time, and correlated their effects with their phytochemical constituents.

It is worth focusing on elucidating pure compounds and identifying their mechanism(s) of action.

SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-021-03423-x.

Mehrbod, Parvaneh,Safari, Hanieh,Mollai, Zeinab,Fotouhi, Fatemeh,Mirfakhraei, Yasaman,Entezari, Hanieh,Goodarzi, Saied,Tofighi, Zahra, 2021, Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus, BioMed Central

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