Documentdetail
ID kaart
oai:pubmedcentral.nih.gov:1077...
Onderwerp
MethodAuteur
Chen, Yefei Wang, Jingyi Liu, Jing Lin, Jianbang Lin, Yunping Nie, Jinyao Yue, Qi Deng, Chunshan Qi, Xiaofei Li, Yuantao Dai, Ji Lu, ZhonghuaLangue
enEditor
Springer Nature Singapore
Categorie
Neuroscience Bulletin
Jaar
2023
vermelding datum
08-01-2025
Trefwoorden
aav functional retrograde aav-dj8r neurons projectionBeschrijving
Retrograde adeno-associated viruses (AAVs) are capable of infecting the axons of projection neurons and serve as a powerful tool for the anatomical and functional characterization of neural networks.
However, few retrograde AAV capsids have been shown to offer access to cortical projection neurons across different species and enable the manipulation of neural function in non-human primates (NHPs).
Here, we report the development of a novel retrograde AAV capsid, AAV-DJ8R, which efficiently labeled cortical projection neurons after local administration into the striatum of mice and macaques.
In addition, intrastriatally injected AAV-DJ8R mediated opsin expression in the mouse motor cortex and induced robust behavioral alterations.
Moreover, AAV-DJ8R markedly increased motor cortical neuron firing upon optogenetic light stimulation after viral delivery into the macaque putamen.
These data demonstrate the usefulness of AAV-DJ8R as an efficient retrograde tracer for cortical projection neurons in rodents and NHPs and indicate its suitability for use in conducting functional interrogations.
SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12264-023-01091-0.
Chen, Yefei,Wang, Jingyi,Liu, Jing,Lin, Jianbang,Lin, Yunping,Nie, Jinyao,Yue, Qi,Deng, Chunshan,Qi, Xiaofei,Li, Yuantao,Dai, Ji,Lu, Zhonghua, 2023, A Novel Retrograde AAV Variant for Functional Manipulation of Cortical Projection Neurons in Mice and Monkeys, Springer Nature Singapore
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