Inhibitory Effect of Apolipoprotein A-I on Eosinophils in Allergic Rhinitis in vitro and in vivo

PURPOSE: Eosinophils have pivotal roles in the development of allergic rhinitis (AR) through the release of cytotoxic substances.

Apolipoprotein A-I (Apo-AI) exhibits a strong inhibitory effect on eosinophil infiltration in allergic diseases.

Nevertheless, the precise impact of Apolipoprotein A-I on eosinophils remains uncertain.

METHODS: Our study recruited a total of 15 AR children and 15 controls.

The correlation between Apo-AI expression and the counts of blood eosinophils was examined.

Flow cytometry was employed to assess the role of Apo-AI in eosinophil apoptosis and adhesion.

The Transwell system was performed to conduct the migration assay.

An animal model using AR mice was established to test the effect of Apo-AI on eosinophils.

RESULTS: Serum Apo-AI were negatively related to eosinophils counts and eosinophil chemotactic protein levels in AR.

Apo-AI exerts a pro-apoptotic effect while also impeding the processes of adhesion, migration, and activation of eosinophils.

The apoptosis triggered by Apo-AI was facilitated through the phosphoinositide 3-kinase (PI3K) pathway.

The chemotaxis and activation of eosinophils, which are influenced by Apolipoprotein A-I, are regulated through the PI3K and MAPK signaling pathways.

Apo-AI treated mice presented with decreased blood and nasal eosinophilic inflammation as well as down-regulated eosinophil related cytokines.

CONCLUSION: Our findings provide confirmation that Apo-AI exhibits inhibitory effects on the function of eosinophils in allergic rhinitis.

This suggests that Apo-AI holds potential as a therapeutic target for future treatment strategies.