oai:pubmedcentral.nih.gov:6838...
Elsevier
EBioMedicine
2019
1/16/2023
BACKGROUND: Both idiopathic and familial Parkinson's disease are associated with mitochondrial dysfunction.
Mitochondria have their own mitochondrial DNA (mtDNA) and previous studies have reported that the release of mtDNA is a biomarker of Parkinson's disease.
METHODS: We have now investigated the relationship between mtDNA replication, transcription and release in fibroblasts from patients with idiopathic (iPD) and Leucine-rich repeat kinase 2(G2019S) -associated Parkinson's disease (LRRK2-PD), using Selfie-digital PCR, a method that allows absolute quantification of mtDNA genomes and transcripts.
FINDINGS: In comparison with healthy controls, we found that fibroblasts from patients with iPD or LRRK2-PD had a high amount of mitochondrial 7S DNA along with a low mtDNA replication rate that was associated with a reduction of cf-mtDNA release.
Accumulation of 7S DNA in iPD and LRRK2-PD fibroblasts was related with an increase in H-strand mtDNA transcription.
INTERPRETATION: These results show that 7S DNA accumulation, low mtDNA replication, high H-strand transcription, and low mtDNA release compose a pattern of mtDNA dysfunction shared by both iPD and LRRK2-PD fibroblasts.
Moreover, these results suggest that the deregulation of the genetic switch formed by 7SDNA that alternates between mtDNA replication and transcription is a fundamental pathophysiological mechanism in both idiopathic and monogenic Parkinson's disease.
Podlesniy, Petar,Puigròs, Margalida,Serra, Núria,Fernández-Santiago, Rubén,Ezquerra, Mario,Tolosa, Eduardo,Trullas, Ramon, 2019, Accumulation of mitochondrial 7S DNA in idiopathic and LRRK2 associated Parkinson's disease, Elsevier