Dokumentdetails
ID
doi:10.1186/s12866-022-02750-4...
Autor
Ducret, Verena Gonzalez, Diego Leoni, Sara Valentini, Martina Perron, KarlLangue
enEditor
BioMed Central
Kategorie
Mycology
Jahr
2023
Auflistungsdatum
08.03.2023
Schlüsselwörter
zinc homeostasis zur 5’-utr ... export zinc aeruginosa system regulation import zn zurZusammenfassung
The control of cellular zinc (Zn) concentrations by dedicated import and export systems is essential for the survival and virulence of Pseudomonas aeruginosa.
The transcription of its many Zn transporters is therefore tightly regulated by a known set of transcription factors involved in either the import or the export of Zn.
In this work, we show that the Zur protein, a well-known repressor of Zn import, plays a dual role and functions in both import and export processes.
In a situation of Zn excess, Zur represses Zn entry, but also activates the transcription of czcR , a positive regulator of the Zn export system.
To achieve this, Zur binds at two sites, located by DNA footprinting in the region downstream the czcR transcription start site.
In agreement with this regulation, a delay in induction of the efflux system is observed in the absence of Zur and Zn resistance is reduced.
The discovery of this regulation highlights a new role of Zur as global regulator of Zn homeostasis in P. aeruginosa disclosing an important link between Zur and zinc export.
Ducret, Verena,Gonzalez, Diego,Leoni, Sara,Valentini, Martina,Perron, Karl, 2023, A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa, BioMed Central
