Dokumentdetails
ID
doi:10.1186/s12866-023-02924-8...
Autor
Peñas-Utrilla, Daniel Sanz, Amadeo Catalán, Pilar Veintimilla, Cristina Alcalá, Luis Alonso, Roberto Muñoz, Patricia Pérez-Lago, Laura García de Viedma, Darío on behalf of the Gregorio Marañón Microbiology-ID COVID 19 Study GroupLangue
enEditor
BioMed Central
Kategorie
Mycology
Jahr
2023
Auflistungsdatum
19.07.2023
Schlüsselwörter
covid-19 cepheid xpert c29200t detection impairment n gene ct xpert snp pandemic sars-cov-2Zusammenfassung
Background COVID-19 diagnosis lies on the detection of SARS-CoV-2 on nasopharyngeal specimens by RT-PCR.
The Xpert-Xpress SARS-CoV-2 assay provides results in less than one hour from specimen reception, which makes it suitable for clinical/epidemiological circumstances that require faster responses.
The analysis of a COVID-19 outbreak suspected in the neonatology ward from our institution showed that the Ct values obtained for the targeted genes in the Xpert assay were markedly different within each specimen (N Ct value > 20 cycles above the E Ct value).
Results We identified the mutation C29200T in the N gene as responsible for an impairment in the N gene amplification by performing whole genome sequencing of the specimens involved in the outbreak (Omicron variant).
Subsequently, a retrospective analysis of all specimens sequenced in our institution allowed us to identify the same SNP as responsible for similar impairments in another 12 cases (42% of the total cases reported in the literature).
Finally, we found that the same SNP emerged in five different lineages independently, throughout almost all the COVID-19 pandemic.
Conclusions We demonstrated for the first time the impact of this SNP on the Xpert assay, when harbored by new Omicron variants.
We extend our observation period throughout almost all the COVID-19 pandemic, offering the most updated observations of this phenomenon, including sequences from the seventh pandemic wave, until now absent in the reports related to this issue.
Continuous monitoring of emerging SNPs that could affect the performance of the most commonly used diagnostic tests, is required to redesign the tests to restore their correct performance.
Peñas-Utrilla, Daniel,Sanz, Amadeo,Catalán, Pilar,Veintimilla, Cristina,Alcalá, Luis,Alonso, Roberto,Muñoz, Patricia,Pérez-Lago, Laura,García de Viedma, Darío,on behalf of the Gregorio Marañón Microbiology-ID COVID 19 Study Group, 2023, A mutation responsible for impaired detection by the Xpert SARS-CoV-2 assay independently emerged in different lineages during the SARS-CoV-2 pandemic, BioMed Central
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