LABS: linear amplification-based bisulfite sequencing for ultrasensitive cancer detection from cell-free DNA

Cui, Xiao-Long; Nie, Ji; Zhu, Houxiang; Kowitwanich, Krissana; Beadell, Alana V.; West-Szymanski, Diana C.; Zhang, Zhou; Dougherty, Urszula; Kwesi, Akushika; Deng, Zifeng; Li, Yan; Meng, Danqing; Roggin, Kevin; Barry, Teresa; Owyang, Ryan; Fefferman, Ben; Zeng, Chang; Gao, Lu; Zhao, Carolyn W. T.; Malina, Yuri; Wei, Jiangbo; Weigert, Melanie; Kang, Wenjun; Goel, Ajay; Chiu, Brian C.-H.; Bissonnette, Marc; Zhang, Wei; Chen, Mengjie; He, Chuan

doi:10.1186/s13059-024-03262-2

LABS: linear amplification-based bisulfite sequencing for ultrasensitive cancer detection from cell-free DNA

detalle del documento

IDENTIFICACIÓN

doi:10.1186/s13059-024-03262-2...

Autor

Cui, Xiao-Long Nie, Ji Zhu, Houxiang Kowitwanich, Krissana Beadell, Alana V. West-Szymanski, Diana C. Zhang, Zhou Dougherty, Urszula Kwesi, Akushika Deng, Zifeng Li, Yan Meng, Danqing Roggin, Kevin Barry, Teresa Owyang, Ryan Fefferman, Ben Zeng, Chang Gao, Lu Zhao, Carolyn W. T. Malina, Yuri Wei, Jiangbo Weigert, Melanie Kang, Wenjun Goel, Ajay Chiu, Brian C.-H. Bissonnette, Marc Zhang, Wei Chen, Mengjie He, Chuan

Langue

Editor

BioMed Central

Categoría

Life Sciences

Año

2024

fecha de cotización

19/6/2024

Palabras clave

linear amplification bisulfite sequencing low-input dna cell-free dna cancer detection labs cancer dna

Métrico

Resumen

Methylation-based liquid biopsies show promises in detecting cancer using circulating cell-free DNA; however, current limitations impede clinical application.

Most assays necessitate substantial DNA inputs, posing challenges.

Additionally, underrepresented tumor DNA fragments may go undetected during exponential amplification steps of traditional sequencing methods.

Here, we report linear amplification-based bisulfite sequencing (LABS), enabling linear amplification of bisulfite-treated DNA fragments in a genome-wide, unbiased fashion, detecting cancer abnormalities with sub-nanogram inputs.

Applying LABS to 100 patient samples revealed cancer-specific patterns, copy number alterations, and enhanced cancer detection accuracy by identifying tissue-of-origin and immune cell composition.

Cui, Xiao-Long,Nie, Ji,Zhu, Houxiang,Kowitwanich, Krissana,Beadell, Alana V.,West-Szymanski, Diana C.,Zhang, Zhou,Dougherty, Urszula,Kwesi, Akushika,Deng, Zifeng,Li, Yan,Meng, Danqing,Roggin, Kevin,Barry, Teresa,Owyang, Ryan,Fefferman, Ben,Zeng, Chang,Gao, Lu,Zhao, Carolyn W. T.,Malina, Yuri,Wei, Jiangbo,Weigert, Melanie,Kang, Wenjun,Goel, Ajay,Chiu, Brian C.-H.,Bissonnette, Marc,Zhang, Wei,Chen, Mengjie,He, Chuan, 2024, LABS: linear amplification-based bisulfite sequencing for ultrasensitive cancer detection from cell-free DNA, BioMed Central