Détail du document
Identifiant
doi:10.1007/s42770-023-01079-y...
Auteur
Abdella, Bahaa Youssif, Asmaa Mohamed Sabry, Soraya A. Ghozlan, Hanan A.Langue
enEditeur
Springer
Catégorie
Mycology
Année
2023
Date de référencement
09/08/2023
Mots clés
lipase cold-adapted plackett-burman optimization psychrotolerantRésumé
Cold-active lipases are presently employed extensively in the detergent, chemical intermediate, fine chemical, food, and pharmaceutical industries.
Seven cold-adaptive bacteria were isolated from the Mediterranean Sea near Alexandria, Egypt, and tested for their ability to produce cold-active lipase, with the highest activity at 10 °C.
The most potent isolate was Pseudomonas sp.
A6.
To determine the most important variables, the bacterium was exposed to a necessary medium component and environmental factor screening using a single factor-at-a-time approach, followed by a multifactorial Plackett-Burman design strategy.
After purification and characterization, the optimal activity levels for the cold-active lipase were figured out.
Inoculation of Pseudomonas A6 under near optimum conditions using medium consisting of (g/L) peptone 7.14; soybean oil 7.5% (v/v); K_2HPO_4, 0.4; MgSO_4, 0.1; glucose 2; pH 8; and temperature 10 °C led to a maximum lipase activity anticipated to be 23.36 U/mL.
Purified lipase showed the best activity and thermal stability at a pH of 8 and a temperature of 10 °C.
The Pseudomonas A6 lipase tolerated the monovalent ions, while greater valence ions did not.
Abdella, Bahaa,Youssif, Asmaa Mohamed,Sabry, Soraya A.,Ghozlan, Hanan A., 2023, Production, purification, and characterization of cold-active lipase from the psychrotroph Pseudomonas sp. A6, Springer
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