Détail du document
Identifiant
doi:10.1186/s12866-023-03143-x...
Auteur
Motamedi, Hamid Abiri, Ramin Salari, Farhad Jalili, Cyrus Alvandi, AmirhoushangLangue
enEditeur
BioMed Central
Catégorie
Mycology
Année
2023
Date de référencement
20/12/2023
Mots clés
ureb caga sirna ... caga sirna urebRésumé
Background Two important virulence factors, urease and cagA, play an important role in Helicobacter pylori ( H. pylori ) gastric cancer.
Aim of this study was to investigate the expression level and function of ureB and cagA using small interfering RNAs (siRNA).
Methods SS1 strain of H. pylori was considered as host for natural transformation.
siRNA designed for ureB and cagA genes were inserted in pGPU6/GFP/Neo siRNA plasmid vector to evaluate using phenotypic and genotypic approaches.
Then, qPCR was performed for determining inhibition rate of ureB and cagA gene expression.
Results The expression levels of siRNA-ureB and siRNA-cagA in the recombinant strain SS1 were reduced by about 5000 and 1000 fold, respectively, compared to the native H. pylori strain SS1.
Also, preliminary evaluation of siRNA-ureB in vitro showed inhibition of urea enzyme activity.
These data suggest that siRNA may be a powerful new tool for gene silencing in vitro, and for the development of RNAi-based anti- H. pylori therapies.
Conclusion Our results show that targeting ureB and cagA genes with siRNA seems to be a new strategy to inhibit urease enzyme activity, reduce inflammation and colonization rate.
Motamedi, Hamid,Abiri, Ramin,Salari, Farhad,Jalili, Cyrus,Alvandi, Amirhoushang, 2023, Reduction of UreB and CagA expression level by siRNA construct in Helicobacter pylori strain SS1, BioMed Central
Machine learning and EEG can classify passive viewing of discrete categories of visual stimuli but not the observation of pain
eeg classify neutral ml participants non-pain expressions images scenes pain
