Détail du document
Identifiant

doi:10.1186/s12866-024-03283-8...

Auteur
Ganjo, Aryan R. Balaky, Salah Tofik Jalal Mawlood, Ahang Hasan Smail, Sakar B. Shabila, Nazar P.
Langue
en
Editeur

BioMed Central

Catégorie

Mycology

Année

2024

Date de référencement

17/04/2024

Mots clés
antibiotic resistance cccp covid-19 ... efflux pumps related meropenem increased bacterial patients covid-19 secondary coli isolates pump resistance levofloxacin
Métrique

Résumé

Background Escherichia coli ( E. coli ) is a multidrug resistant opportunistic pathogen that can cause secondary bacterial infections in patients with COVID-19.

This study aimed to determine the antimicrobial resistance profile of E. coli as a secondary bacterial infection in patients with COVID-19 and to assess the prevalence and characterization of genes related to efflux pumps and porin.

Methods A total of 50 nonduplicate E. coli isolates were collected as secondary bacterial infections in COVID-19 patients.

The isolates were cultured from sputum samples.

Confirmation and antibiotic susceptibility testing were conducted by Vitek 2.

PCR was used to assess the prevalence of the efflux pump and porin-related genes in the isolates.

The phenotypic and genotypic evolution of antibiotic resistance genes related to the efflux pump was evaluated.

Results The E. coli isolates demonstrated high resistance to ampicillin (100%), cefixime (62%), cefepime (62%), amoxicillin-clavulanic acid (60%), cefuroxime (60%), and ceftriaxone (58%).

The susceptibility of E. coli to ertapenem was greatest (92%), followed by imipenem (88%), meropenem (86%), tigecycline (80%), and levofloxacin (76%).

Regarding efflux pump gene combinations, there was a significant association between the acrA gene and increased resistance to levofloxacin, between the acrB gene and decreased resistance to meropenem and increased resistance to levofloxacin, and between the ompF and ompC genes and increased resistance to gentamicin.

Conclusions The antibiotics ertapenem, imipenem, meropenem, tigecycline, and levofloxacin were effective against E. coli in patients with COVID-19.

Genes encoding efflux pumps and porins, such as acrA, acrB, and outer membrane porins , were highly distributed among all the isolates.

Efflux pump inhibitors could be alternative antibiotics for restoring tetracycline activity in E. coli isolates.

Ganjo, Aryan R.,Balaky, Salah Tofik Jalal,Mawlood, Ahang Hasan,Smail, Sakar B.,Shabila, Nazar P., 2024, Characterization of genes related to the efflux pump and porin in multidrug-resistant Escherichia coli strains isolated from patients with COVID-19 after secondary infection, BioMed Central

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