Détail du document
Identifiant
oai:pubmedcentral.nih.gov:8208...
Sujet
Research ArticlesAuteur
Barroso, Shana Priscila Coutinho Vicente dos Santos, Ana Clara Souza dos Santos, Patrícia dos Santos Silva Couceiro, José Nelson Fernandes Ferreira, Davis Nico, Dirlei Morrot, Alexandre Lima Silva, Jerson Cheble de Oliveira, AndreaLangue
enEditeur
Microbiology Society
Catégorie
Access Microbiology
Année
2021
Date de référencement
08/11/2022
Mots clés
virus hydrostatic inactivation influenza pressureRésumé
Vaccines are a recommended strategy for controlling influenza A infections in humans and animals.
Here, we describe the effects of hydrostatic pressure on the structure, morphology and functional characteristics of avian influenza A H3N8 virus.
The effect of hydrostatic pressure for 3 h on H3N8 virus revealed that the particles were resistant to this condition, and the virus displayed only a discrete conformational change.
We found that pressure of 3 kbar applied for 6 h was able to inhibit haemagglutination and infectivity while virus replication was no longer observed, suggesting that full virus inactivation occurred at this point.
However, the neuraminidase activity was not affected at this approach suggesting the maintenance of neutralizing antibody epitopes in this key antigen.
Our data bring important information for the area of structural virology of enveloped particles and support the idea of applying pressure-induced inactivation as a tool for vaccine production.
Barroso, Shana Priscila Coutinho,Vicente dos Santos, Ana Clara,Souza dos Santos, Patrícia,dos Santos Silva Couceiro, José Nelson,Fernandes Ferreira, Davis,Nico, Dirlei,Morrot, Alexandre,Lima Silva, Jerson,Cheble de Oliveira, Andrea, 2021, Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach, Microbiology Society
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