Détail du document
Identifiant
oai:pubmedcentral.nih.gov:1005...
Sujet
ArticleAuteur
Martin, Rebeca San Das, Priyojit Xue, Tianchun Brown, Morgan Rose Reis Marques, Renata Dos Essington, Michael Gonzalez, Adrian McCord, Rachel PattonLangue
enEditeur
Cold Spring Harbor Laboratory
Catégorie
biorxiv
Année
2023
Date de référencement
05/04/2023
Mots clés
surfaces microenvironment cell cancer calcium prostate boneRésumé
Bone metastasis remains one of the biggest challenges in the treatment of prostate cancer, and other solid tumors such as breast, lung, and colon.
Modeling a complex microenvironment in-vitro, such as the bone niche, requires interrogation of cell-cell interactions, specific extracellular matrix proteins and a high calcium environment.
Here, we present a fast and cost-effective system in which commercially available, non-adhesive, cell culture vessels are coated with amorphous calcium phosphate (ACP) as a surrogate for bone matrix.
We further present modified protocols for subculturing cells, as well as nucleic acid and protein collection in high calcium samples.
We find that prostate epithelial cell lines show increased adhesion and proliferation when cultured in these surfaces, as well as independence from androgen starvation.
We observe gene expression changes on ACP surfaces in early adenocarcinoma cell lines which may reflect alterations relevant to prostate cancer progression.
SUMMARY STATEMENT: To model the role of calcium in the microenvironment of the metastatic bone niche, we developed a cost-effective way to coat cell culture vessels in bioavailable calcium, and show that it has an effect on prostate cancer cell survival
Martin, Rebeca San,Das, Priyojit,Xue, Tianchun,Brown, Morgan Rose,Reis Marques, Renata Dos,Essington, Michael,Gonzalez, Adrian,McCord, Rachel Patton, 2023, Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer , Cold Spring Harbor Laboratory
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