Détail du document
Identifiant
oai:HAL:hal-03278552v1
Sujet
influenza vaccine recombinant nucleoprotein protection cellular immunity CD8+ T-cells [SDV.IMM]Life Sciences [q-bio]/Imm...Auteur
del Campo, Judith Bouley, Julien Chevandier, Marion Rousset, Carine Haller, Marjorie Indalecio, Alice Guyon-Gellin, Delphine Le Vert, Alexandre Hill, Fergal Djebali, Sophia Leverrier, Yann Marvel, Jacqueline Combadière, Béhazine Nicolas, FlorenceLangue
enEditeur
HAL CCSD;Frontiers
Catégorie
CNRS - Centre national de la recherche scientifique
Année
2021
Date de référencement
29/09/2023
Mots clés
vaccine induces recombinant np nucleoprotein lung influenza cd8+ protection t-cellsRésumé
International audience; Tissue-resident memory (TRM) CD8+ T-cells play a crucial role in the protection against influenza infection but remain difficult to elicit using recombinant protein vaccines.
OVX836 is a recombinant protein vaccine, obtained by the fusion of the DNA sequence of the influenza A nucleoprotein (NP) to the DNA sequence of the OVX313 heptamerization domain.
We previously demonstrated that OVX836 provides broad-spectrum protection against influenza viruses.
Here, we show that OVX836 intramuscular (IM) immunization induces higher numbers of NP-specific IFNg-producing CD8+ T-cells in the lung, compared to mutant NP (NPm) and wild-type NP (NPwt), which form monomeric and trimeric structures, respectively.
OVX836 induces cytotoxic CD8+ T-cells and high frequencies of lung TRM CD8+ T-cells, while inducing solid protection against lethal influenza virus challenges for at least 90 days.
Adoptive transfer experiments demonstrated that protection against diverse influenza subtypes is mediated by NPspecific CD8+ T-cells isolated from the lung and spleen following OVX836 vaccination.
OVX836 induces a high number of NP-specific lung CD8+ TRM-cells for long-term protection against influenza viruses.
del Campo, Judith,Bouley, Julien,Chevandier, Marion,Rousset, Carine,Haller, Marjorie,Indalecio, Alice,Guyon-Gellin, Delphine,Le Vert, Alexandre,Hill, Fergal,Djebali, Sophia,Leverrier, Yann,Marvel, Jacqueline,Combadière, Béhazine,Nicolas, Florence, 2021, OVX836 Heptameric Nucleoprotein Vaccine Generates Lung Tissue-Resident Memory CD8+ T-Cells for Cross-Protection Against Influenza, HAL CCSD;Frontiers
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