Lipocalin 2 (LCN2) Knockdown Regulates Treg/Th17 Balance to Improve Asthma in Mice

PURPOSE: Asthma substantially affects the quality of life and health of children.

Lipocalin 2 (LCN2) is an immune-related protein, which is predicted to be highly expressed in asthma.

Here, we investigated the role of LCN2 in ovalbumin (OVA)-induced asthma mouse model.

METHODS: We knocked down LCN2 in an asthma mouse model and performed histopathological analysis using hematoxylin and eosin (H&E) staining assay.

Differentiated cells were assessed using Diff-Quick staining assay.

We investigated the regulatory T (Treg) cell/ T helper 17 (Th17) cell balance using flow cytometry and enzyme-linked immunosorbent assay (ELISA).

Inflammatory factors were measured using quantitative real-time reverse transcription PCR (qRT-PCR).

The involved pathways were assessed using Western blotting.

RESULTS: LCN2 was upregulated in patients with asthma.

OVA promoted pathological deterioration in the lungs, increased IgE levels in the plasma, and elevated the number of differentiated inflammatory cells, whereas LCN2 knockdown abrogated the OVA-induced effects.

Additionally, the Treg/Th17 imbalance and increased inflammatory cytokine levels were improved by LCN2 knockdown in OVA-treated mice.

Moreover, LCN2 knockdown reversed the activation of the janus kinase (JNK) pathway.

CONCLUSION: LCN2 knockdown improved the Treg/Th17 balance, alleviated inflammation, and inactivated the JNK pathway in OVA-induced asthma mouse model, suggesting that LCN2 may be a novel therapeutic target for asthma in children.